Dépôt numérique

Functional responsiveness of in vitro-aged human neutrophils

Morin-Genest, Julie; Saafane, Abdelaziz et Girard, Denis ORCID logoORCID: https://orcid.org/0000-0002-3342-5027 (2023). Functional responsiveness of in vitro-aged human neutrophils Cellular Immunology , vol. 390 , nº 104739. pp. 1-8. DOI: 10.1016/j.cellimm.2023.104739.

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Elimination of apoptotic neutrophils by macrophages is as a major step for the resolution of inflammation. However, the fate and the cellular functionality of neutrophils aged in the absence of macrophages are not well documented. Herein, freshly isolated human neutrophils were aged for several days in vitro and then stimulated with agonists for determining their cell responsiveness. In vitro-aged neutrophils were still able to generate reactive oxygen species after 48 h, exert phagocytosis after 72 h, and increase their adhesion onto a cell substratum after 48 h. These data demonstrate that a portion of neutrophils cultivated for several days in vitro are still able to exert biological functions. This opens the possibility that, during inflammation, neutrophils may still respond to agonists, a condition that is likely to occur in vivo when they are not efficiently eliminated by efferocytosis.

Type de document: Article
Mots-clés libres: Inflammation; Neutrophils; Aging; Apoptosis
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 09 déc. 2023 18:25
Dernière modification: 09 déc. 2023 18:25
URI: https://espace.inrs.ca/id/eprint/13655

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