Dépôt numérique

Reproductive toxicity of emerging plasticizers, flame-retardants and bisphenols, using culture of the rat fetal testis

Tardif, Sarah; Rwigemera, Arlette; Létourneau, Natasha; Robaire, Bernard et Delbès, Géraldine ORCID logoORCID: https://orcid.org/0000-0002-9169-1075 (2023). Reproductive toxicity of emerging plasticizers, flame-retardants and bisphenols, using culture of the rat fetal testis Biology of Reproduction , vol. - . DOI: 10.1093/biolre/ioad018. (Sous Presse)

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The use of bis (2-ethylhexyl) phthalate (DEHP), 2,2'4,4'-tetrabromodiphenyl ether (BDE-47) and bisphenol A (BPA), as plasticizers, flame-retardant and epoxy resins, respectively, has been regulated due to their endocrine disrupting chemical (EDC) activities. Replacements for these chemicals are found in the environment and human matrices, yet the EDC activity of these emerging contaminants is poorly characterized. We compared the effects of legacy chemicals with those of their replacements using fetal rat testis organ culture. Fetal testes sampled at gestation day 15 were grown ex vivo and the impact was evaluated after a 3-day exposure to 10 μM of each legacy chemical, two BPA analogs (BPM and BPTMC), three replacements for DEHP/MEHP (TXIB, DINP, DIDA) or two replacements for BDE-47 (TBEP, IPPP). Using culture media, we showed that only BPA and MEHP significantly decrease basal testosterone secretions after 24 h of treatment while BPM and BPTMC have the opposite effect. LH-stimulated testosterone was reduced by BPA and MEHP but increased by BPTMC. After exposure, testes were used for immunofluorescent staining of germ cells, Sertoli cells and Leydig cells. Interestingly, the effect of BPM and BPTMC on testosterone secretion was associated with a significant increase in Leydig cell density and surface area. A decrease in germ cell density was observed only after treatment with MEHP or BDE-47. MEHP significantly decreased Sertoli cell proliferation. These studies show that some replacement chemicals can affect testicular function while others appear to show little toxicity in this model. These findings provide essential information regarding the need for their regulation.

Type de document: Article
Mots-clés libres: Fetal testis; Leydig cells; Sertoli cells; Bisphenols; Flame retardants; Germ cells; Organ culture; Plasticizers; Substitutes; Testosterone
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 25 juill. 2023 04:24
Dernière modification: 25 juill. 2023 04:24
URI: https://espace.inrs.ca/id/eprint/13482

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