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Cap-dependent translation initiation monitored in living cells


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Gandin, Valentina; English, Brian P; Freeman, Melanie; Leroux, Louis-Philippe; Preibisch, Stephan; Walpita, Deepika; Jaramillo, Maritza ORCID logoORCID: https://orcid.org/0000-0002-1910-5684 et Singer, Robert H (2022). Cap-dependent translation initiation monitored in living cells Nature Communications , vol. 13 , nº 6558. pp. 1-15. DOI: 10.1038/s41467-022-34052-8.

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mRNA translation is tightly regulated to preserve cellular homeostasis. Despite extensive biochemical, genetic, and structural studies, a detailed understanding of mRNA translation regulation is lacking. Imaging methodologies able to resolve the binding dynamics of translation factors at single-cell and single-mRNA resolution were necessary to fully elucidate regulation of this paramount process. Here live-cell spectroscopy and single-particle tracking were combined to interrogate the binding dynamics of endogenous initiation factors to the 5'cap. The diffusion of initiation factors (IFs) changed markedly upon their association with mRNA. Quantifying their diffusion characteristics revealed the sequence of IFs assembly and disassembly in cell lines and the clustering of translation in neurons. This approach revealed translation regulation at high spatial and temporal resolution that can be applied to the formation of any endogenous complex that results in a measurable shift in diffusion.

Type de document: Article
Mots-clés libres: Translation; Cell biology
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 09 déc. 2023 17:36
Dernière modification: 09 déc. 2023 17:36
URI: https://espace.inrs.ca/id/eprint/13386

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