Dépôt numérique

Influence of Growth Parameters on Bacteriocin-Like Inhibitory Substances (BLIS) Production by Lactic Acid Bacteria

Foudjing, Gildas Gaelle Domguia; Sarmast, Elham; Allahdad, Zahra; Salmieri, Stéphane et Lacroix, Monique ORCID logoORCID: https://orcid.org/0000-0002-2042-4033 (2022). Influence of Growth Parameters on Bacteriocin-Like Inhibitory Substances (BLIS) Production by Lactic Acid Bacteria Letters in Applied Microbiology , vol. 76 , nº ovac013. pp. 1-10. DOI: 10.1093/lambio/ovac013.

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This study aimed to investigate the possible production of bacteriocin-like inhibitory substances (BLIS) and optimize it by Lactobacillus sakei and combined Lactobacillus curvatus/Pediococcus acidilactici, lactic acid bacteria used for the meat fermentation. The effect of growth parameters on BLIS production was evaluated by measuring the antibacterial activity of the cell-free supernatant of these bacteria. The stability of the cell-free supernatant of combined L.curvatus/P.acidilactici bacteria was also examined after heat treatment and pH variations. Results showed highest BLIS production by L.curvatus/P.acidilactici was at growth temperatures of 28 degrees C and pH 5, while the optimum condition for production of BLIS by L.sakei was 24 degrees C and pH 6.5. The BLIS production by L. curvatus/P. acidilactici bacteria was greatly influenced by carbon and nitrogen sources. The antimicrobial activity of BLIS in supplemented De Man, Rosa, and Sharpe broth (MRS) was 2044.44 AU/mL, 4.84-fold greater than unsupplemented MRS. None of the carbon sources used in this study affected the BLIS production by the L.sakei, while pea peptone enhanced the antimicrobial activity and showed a value of 188.89 AU/mL, 1.47-fold greater than unsupplemented MRS. The characterization of the cell-free supernatant of L. curvatus/P. acidilactici bacteria showed the heat stability and activity of BLIS at pH 2-10.

Type de document: Article
Mots-clés libres: lactic acid bacteria; postbiotic; bacteriocin-like inhibitory substances; growth parameters; antimicrobial activity
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 17 oct. 2023 14:58
Dernière modification: 17 oct. 2023 14:58
URI: https://espace.inrs.ca/id/eprint/13381

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