Dépôt numérique

DNA Methylation Programming and Environmental Influences During Rat Gametogenesis

El Omri-Charai, Rizlane; Gilbert, Isabelle; Rwigemera, Arlette; Desmarais, Rebecka; Ghinet, Mariana Gabriela; Prunier, Julien; Langford, Alexandra; Sloboda, Deborah; McGraw, Serge; Robert, Claude; Boissonneault, Guylain et Delbès, Géraldine ORCID logoORCID: https://orcid.org/0000-0002-9169-1075 (2022). DNA Methylation Programming and Environmental Influences During Rat Gametogenesis In: 13th International Conference on Environmental Mutagens, August 27 - September 01, 2022, Ottawa Canada.

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Early embryogenesis and germline establishment are the two main developmental windows where DNA methylation (DNAme) reprogramming occurs. The concept of the developmental origins of health and disease (DOHaD) supports that the in-utero environment may impact such reprogramming. Here, we questioned the immediate effect of a maternal highfat diet (HFD) on early reprogramming of rat primordial germ cells (PGCs) and investigated the dynamics of DNAme in the male germline, identifying potential windows of sensitivity. Using a targeted DNAme capture analysis, we first observed that male and female PGCs from HFD-treated mothers exhibited a slight global hypomethylation at gestational day 16 (GD16), suggesting an advancement of DNAme erasure. Interestingly, HFD-induced differentially methylated regions (DMRs) were sex-specific, yet mostly in intronic and intergenic regions in both sexes. We also found that global DNAme level were higher in male than female PGCs at GD16. Further targeted mapping of DNAme was done in the control male germline from 9 cell populations at key steps of gametogenesis (perinatal stages, spermiogenesis, and spermatozoa). We have identified that GD18 is the stage at which DNAme reached its lowest level in the rat and described the kinetics of de novo DNAme according to the different regions of the genome. Furthermore, we were able to identify specific kinetics of DNAme variations during spermiogenesis leading to the DNAme pattern of mature spermatozoa. Together, these data allow the identification of potential windows of DNAme sensitivity to the environment, in specific regions of the genome, throughout gametogenesis.

Type de document: Document issu d'une conférence ou d'un atelier
Informations complémentaires: Présentation orale P35 Environmental and Molecular Mutagenesis 67:, pp.87 Platform 6: Epigenomics and Heritable Effects
Mots-clés libres: -
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 04 janv. 2024 07:56
Dernière modification: 04 janv. 2024 07:56
URI: https://espace.inrs.ca/id/eprint/13372

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