El Omri-Charai, Rizlane; Gilbert, Isabelle; Rwigemera, Arlette; Desmarais, Rebecka; Ghinet, Mariana Gabriela; Prunier, Julien; Langford, Alexandra; Sloboda, Deborah; McGraw, Serge; Robert, Claude; Boissonneault, Guylain et Delbès, Géraldine 
ORCID: https://orcid.org/0000-0002-9169-1075
(2022).
DNA Methylation Programming and Environmental Influences During Rat Gametogenesis
In: 13th International Conference on Environmental Mutagens, August 27 - September 01, 2022, Ottawa Canada.
Résumé
Early embryogenesis and germline establishment are
the two main developmental windows where DNA
methylation (DNAme) reprogramming occurs. The
concept of the developmental origins of health and
disease (DOHaD) supports that the in-utero
environment may impact such reprogramming. Here,
we questioned the immediate effect of a maternal highfat diet (HFD) on early reprogramming of rat primordial
germ cells (PGCs) and investigated the dynamics of
DNAme in the male germline, identifying potential
windows of sensitivity. Using a targeted DNAme
capture analysis, we first observed that male and
female PGCs from HFD-treated mothers exhibited a
slight global hypomethylation at gestational day 16
(GD16), suggesting an advancement of DNAme
erasure. Interestingly, HFD-induced differentially methylated regions (DMRs) were sex-specific, yet
mostly in intronic and intergenic regions in both sexes.
We also found that global DNAme level were higher in
male than female PGCs at GD16. Further targeted
mapping of DNAme was done in the control male
germline from 9 cell populations at key steps of
gametogenesis (perinatal stages, spermiogenesis, and
spermatozoa). We have identified that GD18 is the
stage at which DNAme reached its lowest level in the
rat and described the kinetics of de novo DNAme
according to the different regions of the genome.
Furthermore, we were able to identify specific kinetics
of DNAme variations during spermiogenesis leading to
the DNAme pattern of mature spermatozoa. Together,
these data allow the identification of potential windows
of DNAme sensitivity to the environment, in specific
regions of the genome, throughout gametogenesis.
| Type de document: | Document issu d'une conférence ou d'un atelier |
|---|---|
| Informations complémentaires: | Présentation orale P35 Environmental and Molecular Mutagenesis 67:, pp.87 Platform 6: Epigenomics and Heritable Effects |
| Mots-clés libres: | - |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 04 janv. 2024 07:56 |
| Dernière modification: | 04 janv. 2024 07:56 |
| URI: | https://espace.inrs.ca/id/eprint/13372 |
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