Dépôt numérique

Differential gene expression and hallmarks of stemness in epithelial cells of the developing rat epididymis

Dufresne, Julie; Gregory, Mary; Pinel, Laurie et Cyr, Daniel G. ORCID logoORCID: https://orcid.org/0000-0002-6566-783X (2022). Differential gene expression and hallmarks of stemness in epithelial cells of the developing rat epididymis Cell and Tissue Research , vol. 389 , nº 2. pp. 327-349. DOI: 10.1007/s00441-022-03634-9.

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Epididymal development can be subdivided into three phases: undifferentiated, a period of differentiation, and expansion. The objectives of this study were (1) to assess gene expression profiles in epididymides, (2) predict signaling pathways, and (3) develop a novel 3D cell culture method to assess the regulation of epididymal development in vitro. Microarray analyses indicate that the largest changes in differential gene expression occurred between the 7- to 18-day period, in which 1452 genes were differentially expressed, while 671 differentially expressed genes were noted between days 18 and 28, and there were 560 differentially expressed genes between days 28 and 60. Multiple signaling pathways were predicted at different phases of development. Pathway associations indicated that in epididymides of 7- to 18-day old rats, there was a significant association of regulated genes implicated in stem cells, estrogens, thyroid hormones, and kidney development, while androgen- and estrogen-related pathways were enriched at other phases of development. Organoids were derived from CD49f + columnar cells from 7-day old rats, while no organoids developed from CD49f(-) cells. Cells cultured in an epididymal basal cell organoid medium versus a commercial kidney differentiation medium supplemented with DHT revealed that irrespective of the culture medium, cells within differentiating organoids expressed p63, AQP9, and V-ATPase after 14 days of culture. The commercial kidney medium resulted in an increase in the number of organoids positive for p63, AQP9, and V-ATPase. Together, these data indicate that columnar cells represent an epididymal stem/progenitor cell population.

Type de document: Article
Mots-clés libres: CD49f; Cell isolation; Cell signaling; Columnar cells; Epididymis; Genomics.
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 09 déc. 2023 16:26
Dernière modification: 09 déc. 2023 16:26
URI: https://espace.inrs.ca/id/eprint/13370

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