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Enzymatic biodegradation of highly p-xylene contaminated soil using cold-active enzymes: A soil column study.

Miri, Saba, Davoodi, Seyyed Mohammadreza, Robert, Thomas, Brar, Satinder Kaur, Martel, Richard ORCID: https://orcid.org/0000-0003-4219-5582 et Rouissi, Tarek (2022). Enzymatic biodegradation of highly p-xylene contaminated soil using cold-active enzymes: A soil column study. Journal of Hazardous Materials , vol. 423 . p. 127099. DOI: 10.1016/j.jhazmat.2021.127099.

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Résumé

Enzymatic bioremediation is a sustainable and environment-friendly method for the clean-up of contaminated soil and water. In the present study, enzymatic bioremediation was designed using cold-active enzymes (psychrozymes) which catalyze oxidation steps of p-xylene biodegradation in highly contaminated soil (initial concentration of 13,000 mg/kg). The enzymes were obtained via co-culture of two psychrophilic Pseudomonas strains and characterized by kinetic studies and tandem LC-MS/MS. To mimic in situ application of enzyme mixture, bioremediation of p-xylene contaminated soil was carried out in soil column (140 mL) tests with the injection (3 pore volume) of different concentrations of enzyme cocktails (X, X/5, and X/10). Enzyme cocktail in X concentration contained about 10 U/mL of xylene monooxygenase (XMO) and 20 U/mL of catechol 2, 3 dioxygenases (C2,3D). X/5 and X/10 correspond to 5x and 10x dilution of enzyme cocktail respectively. The results showed that around 92–94% p-xylene removal was achieved in the treated soil column with enzyme concentration X, X/5 after second enzyme injection. While the p-xylene removal rate obtained by X/10 concentration of enzyme was less than 30% and near to untreated soil column (22.2%). The analysis of microbial diversity and biotoxicity assay (root elongation and seed germination) confirmed the advantage of using enzymes as a green and environmentally friendly approach for decontamination of pollutants with minimal or even positive effects on microbial community and also enrichment of soil after treatment.

Type de document: Article
Mots-clés libres: P-xylene; enzymatic bioremediation; cold-active enzymes; biodiversity; soil column
Centre: Centre Eau Terre Environnement
Date de dépôt: 23 juin 2022 14:51
Dernière modification: 23 juin 2022 14:51
URI: https://espace.inrs.ca/id/eprint/12724

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