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The fungal effector Mlp37347 alters plasmodesmata fluxes and enhances susceptibility to pathogen

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Rahman, Md Saifur, Madina, Mst Hur, Plourde, Mélodie B, Dos Santos, Karen Cristine Gonçalves, Huang, Xiaoqiang, Zhang, Yang, Laliberté, Jean-François et Germain, Hugo (2021). The fungal effector Mlp37347 alters plasmodesmata fluxes and enhances susceptibility to pathogen Microorganisms , vol. 9 , nº 6:1232. p. 1-15. DOI: 10.3390/microorganisms9061232.

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Résumé

Melampsora larici-populina (Mlp) is a devastating pathogen of poplar trees, causing the defoliating poplar leaf rust disease. Genomic studies have revealed that Mlp possesses a repertoire of 1184 small secreted proteins (SSPs), some of them being characterized as candidate effectors. However, how they promote virulence is still unclear. This study investigates the candidate effector Mlp37347’s role during infection. We developed a stable Arabidopsis transgenic line expressing Mlp37347 tagged with the green fluorescent protein (GFP). We found that the effector accumulated exclusively at plasmodesmata (PD). Moreover, the presence of the effector at plasmodesmata favors enhanced plasmodesmatal flux and reduced callose deposition. Transcriptome profiling and a gene ontology (GO) analysis of transgenic Arabidopsis plants expressing the effector revealed that the genes involved in glucan catabolic processes are up-regulated. This effector has previously been shown to interact with glutamate decarboxylase 1 (GAD1), and in silico docking analysis supported the strong binding between Mlp37347 and GAD1 in this study. In infection assays, the effector promoted Hyalonoperospora arabidopsidis growth but not bacterial growth. Our investigation suggests that the effector Mlp37347 targets PD in host cells and promotes parasitic growth. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).

Type de document: Article
Mots-clés libres: Melampsora larici-populina; Mlp37347; Effector; Glutamate Decarboxylase; Plasmodesmata
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 30 juin 2022 14:13
Dernière modification: 30 juin 2022 14:13
URI: https://espace.inrs.ca/id/eprint/12424

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