Dépôt numérique

Radiosensitivity of Feline Calicivirus F9 on Iceberg Lettuce Surface after Combined Treatments with gamma-Radiation

Gobeil, Alexandra; Shankar, Shiv et Lacroix, Monique ORCID logoORCID: https://orcid.org/0000-0002-2042-4033 (2020). Radiosensitivity of Feline Calicivirus F9 on Iceberg Lettuce Surface after Combined Treatments with gamma-Radiation Journal of Food Protection , vol. 83 , nº 12. pp. 2134-2146. DOI: 10.4315/JFP-19-464.

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The surface of iceberg lettuce (Lactuca sativa L.) is favorable to the survival of pathogens such as bacteria, parasites, and viruses such as norovirus. The present study was conducted to investigate the antiviral properties of treatment with cranberry juice (CJ), ozone (O-3), and.-radiation alone or in combination against feline calicivirus (FCV) F9 present on the surface of iceberg lettuce. The lettuce leaves were inoculated with virus suspensions at similar to 6 log TCID50 (50% tissue culture infective dose)/mL and treated with CJ, O-3, and.-radiation alone and in combination during storage at 4 degrees C. The D10-values of 1.21 kGy, 2.23% CJ, and 14.93 ppm of O-3 were obtained when samples were treated with various radiation doses, CJ, and O3, respectively. Relative radiosensitization of FCV-F9 virus on lettuce was 1.20, 1.50, 1.09, and 1.00 after combined CJ treatments of 0.1, 0.25, 0.50, and 1.50%, respectively. Optimum treatments were 5 ppm for 7.5 min for O3, 0.25% CJ, and gamma-radiation at 1.5 kGy when each treatment was used alone. The combination of the three treatments produced the highest reduction of 2.15 log TCID50/mL (from initial inoculation of similar to 7 log TCID50/mL) during 10 days of storage at 48C. Antibacterial properties of treatments and physicochemical quality of lettuce were investigated during 13 days of storage at 4 degrees C. The treatment of lettuce with gamma-radiation alone (1.5 kGy) reduced the total flora by 3 log CFU/g; however, the combination of CJ (0.25%) with irradiation (1.5 kGy) reduced it by similar to 5 log CFU/g after 13 days of storage at 48C. The texture and color of the lettuce treated with the combined treatments changed slightly during storage, and chlorophyll increased by 3.81 mu g/mL after 10 days of storage at 48C. Significant differences in taste and color were observed in lettuce without treatments after 5 days of storage, whereas no difference was observed after the 0.25% CJ or the combined treatments. HIGHLIGHTS Irradiation, CJ, and O-3 combined are more effective against FCV than are single treatments. Irradiation alone and in combined treatments had potent antibacterial activity. Combined treatments did not change the physicochemical and sensorial properties of lettuce. Chlorophyll increased in iceberg lettuce after the combination treatments.

Type de document: Article
Mots-clés libres: Feline calicivirus; Human norovirus; Irradiation; Lettuce; Organic acids; Ozone
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 23 juin 2022 02:27
Dernière modification: 23 juin 2022 02:27
URI: https://espace.inrs.ca/id/eprint/12336

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