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Patterns of apolipoproteins J, D and oxidative markers in circulating extracellular vesicles from MCI and Alzheimer’s patients

Perrotte, Morgane, ben Khedher, Mohamed Raâfet, Haddad, Mohamed, Fulop, Tamas et Ramassamy, Charles . Patterns of apolipoproteins J, D and oxidative markers in circulating extracellular vesicles from MCI and Alzheimer’s patients In: International Society for Extracellular Vesicle (Annual Meeting ISEV2020), 20-22 July 2020, virtuel.

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Résumé

Introduction: Oxidative insults are known to be involved in the pathophysiology of Alzheimer’s disease (AD). We have previously demonstrated that some blood-based redox-signature were associated to the cognitive scores in mild cognitive impairment patients and in AD (Perrotte et al., 2019). The aim of this study was (1) to evidence the presence of some oxidative markers in circulating extracellular vesicles (EVs), and (2) to compare to their plasma levels.

Methods: Plasma samples from healthy, MCI and AD patients were from the Memory Clinic of Sherbrooke (Québec, Canada). AD patients were stratified in three groups (moderate, mild and severe) according to the MMSE and MoCA scores. Total plasma extracellular vesicles (pEVs) were isolated from plasma with the Total Exosome Isolation reagent (Invitrogen™ by Life Technologies Inc.). pEVs were then characterized by electronic microscopy, NTA, DLS and Western Blot. Antioxidants apolipoprotein J, D (apo J, ApoD), the glyoxalase-1 and protein carbonyls were determined by Western blot.

Results: In pEVs, we found that apo D levels were higher in MCI patients but not in AD patients. Protein carbonyls levels were higher later, in pEVs from moderate and severe AD while apo J levels were not different in pEVs from the five groups of patients. In plasma, the pattern of apo J and apo D was different. The levels of apo D was not different in the five groups of patients while apo J levels were elevated in MCI and in all AD groups. Protein carbonyls were higher earlier from mild AD group, earlier than in pEVs. The levels of the detoxifying enzyme glyoxalase-1 were higher in pEVs than in plasma and were significantly decreased in early AD as compared to control subjects and MCI.

Summary/Conclusion: These results demonstrate a differential regulation of redox homeostasis in plasma and in pEVs from AD patients.

Type de document: Document issu d'une conférence ou d'un atelier
Informations complémentaires: Affiche scientifique PT01.03

https://www.eventscribe.com/2020/ISEV/

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Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 14 juill. 2021 15:39
Dernière modification: 14 juill. 2021 15:39
URI: https://espace.inrs.ca/id/eprint/11867

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