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Production of aroma and flavor‐rich fusel alcohols by cheese whey fermentation using the Kluyveromyces marxianus and Debaryomyces hansenii yeasts in monoculture and co‐culture modes.

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Valdez Castillo, Mariana, Tahmasbi, Hamed, Pachapur, Vinayak Laxman, Brar, Satinder Kaur, Vuckovic, Dajana, Sitnikov, Dimitri, Arriaga, Sonia, Blais, Jean‐François ORCID: https://orcid.org/0000-0003-3087-4318 et Avalos Ramirez, Antonio (2021). Production of aroma and flavor‐rich fusel alcohols by cheese whey fermentation using the Kluyveromyces marxianus and Debaryomyces hansenii yeasts in monoculture and co‐culture modes. Journal of Chemical Technology & Biotechnology , vol. 96 , nº 8. p. 2354-2367. DOI: 10.1002/jctb.6763.

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Résumé

BACKGROUND: Whey is one of the main agro-industrial by-products generated in the production of cheese. It is a residue with low pH and high content of organic and inorganic compounds, such as lactose, proteins, and minerals that can be used as nutrients in fermentation. The aim of the present study was to produce biomolecules with aroma and flavor properties by whey fermentation using Kluyveromyces marxianus (KM) and Debaryomyces hansenii (DH). RESULTS: These yeasts produced and accumulated in the culture broth aroma and flavor compounds, the main ones being ethanol, glycerol, propanoic acid, dihydroxyacetone, methionol, isopentanol, and 2-phenylethanol (2PE). This last one was retained as the target biomolecule, because of its potential to be commercialized industrially. Both yeasts were able to metabolize L-phenylalanine (Lphe) to produce 2PE, in monoculture and co-culture modes. When yeasts were used under monoculture mode, KM produced the highest 2PE concentration 82 ± 28 mg/L under aerobic fermentation, with yield of 0.16 ± 0.08 g2PE/gLphe and a productivity of 0.86 ± 0.18 mg2PE/L*h at a fermentation time of 96 h. Whereas in co-culture mode the 2PE yield was 0.38 g2PE/gLphe, twice as high as the maximum yield for monocultures. This yield corresponded to a productivity of 1.93 ± 0.02 mg2PE/L*h. CONCLUSION: The whey fermentation using KM and DH in co-culture mode is technically feasible. The KM yeast is apparently dominant and the co-culture of both yeasts led to increase in the 2PE yield and the productivity. The faster kinetics of KM quickly induced substrate starvation triggering early production and accumulation of 2PE.

Type de document: Article
Mots-clés libres: 2-phenylethanol; aerobic whey fermentation; biotransformation; L-phenylananine; yeasts
Centre: Centre Eau Terre Environnement
Date de dépôt: 15 juill. 2021 15:10
Dernière modification: 19 avr. 2022 04:00
URI: https://espace.inrs.ca/id/eprint/11844

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