Dépôt numérique
RECHERCHER

Prevalence and mechanisms of azole resistance in clinical isolates of Aspergillus section Fumigati species in a Canadian tertiary care centre, 2000 to 2013

Parent-Michaud, Maxime, Dufresne, Philippe J, Fournier, Éric, Folch, Benjamin, Martineau, Christine, Moreira, Sandrine, Doucet, Nicolas ORCID: https://orcid.org/0000-0002-1952-9380, De Repentigny, Louis et Dufresne, Simon F (2019). Prevalence and mechanisms of azole resistance in clinical isolates of Aspergillus section Fumigati species in a Canadian tertiary care centre, 2000 to 2013 Journal of Antimicrobial Chemotherapy , vol. 75 , nº 4. p. 849-858. DOI: 10.1093/jac/dkz534.

Ce document n'est pas hébergé sur EspaceINRS.

Résumé

OBJECTIVES: Azole resistance among Aspergillus fumigatus isolates is a growing concern worldwide. Induction of mutations during azole therapy, environment-acquired mutations caused by azole fungicides and intrinsic resistance of cryptic Fumigati species all contribute to the burden of resistance. However, there is a lack of data in Canada on this emerging threat.

METHODS: To gain insights into the magnitude and mechanisms of resistance, a 14 year collection of Aspergillus section Fumigati comprising 999 isolates from 807 patients at a Montreal hospital was screened for azole resistance, and resistance mechanisms were investigated with the combined use of genome sequencing, 3D modelling and phenotypic efflux pump assays.

RESULTS: Overall azole resistance was low (4/807 patients; 0.5%). A single azole-resistant A. fumigatus sensu stricto strain, isolated from a patient with pulmonary aspergillosis, displayed efflux-pump-mediated resistance. Three patients were colonized or infected with azole-resistant cryptic Fumigati species (one Aspergillus thermomutatus, one Aspergillus lentulus and one Aspergillus turcosus). Evidence is presented that azole resistance is efflux-pump-mediated in the A. turcosus isolate, but not in the A. lentulus and A. thermomutatus isolates.

CONCLUSIONS: Azole resistance is rare in our geographic area and currently driven by cryptic Fumigati species. Continued surveillance of emergence of resistance is warranted.

Type de document: Article
Mots-clés libres: -
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 23 juill. 2021 17:11
Dernière modification: 15 févr. 2022 15:32
URI: https://espace.inrs.ca/id/eprint/11657

Actions (Identification requise)

Modifier la notice Modifier la notice