Modulation of bacterial multicellularity via spatio-specific polysaccharide secretion

Islam, Salim Timo ORCID: https://orcid.org/0000-0001-6853-8446, Vergara Alvarez, israel, Saidi, Fares, Giuseppi, Annick, Vinogradov, Evgeny, Sharma, Gaurav, Espinosa, Léon, Morrone, Castrese, Brasseur, Gael, Guillemot, Jean-Francois, Benarouche, Anais, Bridot, Jean-Luc, Ravicoularamin, Gokulakrishnan, Cagna, Alain, Gauthier, Charles ORCID: https://orcid.org/0000-0002-2475-2050, Singer, Mitchell, Fierobe, Henri-Pierre, Mignot, Tam et Mauriello, Emilia M.F. (2020). Modulation of bacterial multicellularity via spatio-specific polysaccharide secretion PLoS Biology , vol. 18 , nº 6:e30007. p. 1-31. DOI: 10.1371/journal.pbio.3000728.

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Résumé

The development of multicellularity is a key evolutionary transition allowing for differentiation of physiological functions across a cell population that confers survival benefits; among unicellular bacteria, this can lead to complex developmental behaviors and the formation of higher-order community structures. Herein, we demonstrate that in the social delta-proteobacterium Myxococcus xanthus, the secretion of a novel biosurfactant polysaccharide (BPS) is spatially modulated within communities, mediating swarm migration as well as the formation of multicellular swarm biofilms and fruiting bodies. BPS is a type IV pilus (T4P)-inhibited acidic polymer built of randomly acetylated beta-linked tetrasaccharide repeats. Both BPS and exopolysaccharide (EPS) are produced by dedicated Wzx/Wzy-dependent polysaccharide-assembly pathways distinct from that responsible for spore-coat assembly. While EPS is preferentially produced at the lower-density swarm periphery, BPS production is favored in the higher-density swarm interior; this is consistent with the former being known to stimulate T4P retraction needed for community expansion and a function for the latter in promoting initial cell dispersal. Together, these data reveal the central role of secreted polysaccharides in the intricate behaviors coordinating bacterial multicellularity.

Type de document:	Article
Mots-clés libres:	-
Centre:	Centre INRS-Institut Armand Frappier
Date de dépôt:	20 juill. 2021 04:12
Dernière modification:	15 févr. 2022 17:31
URI:	https://espace.inrs.ca/id/eprint/11607

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