Dépôt numérique
RECHERCHER

Hedgehog signaling pathway regulates gene expression profile of epididymal principal cells through the primary cilium

Girardet, Laura; Bernet, Agathe; Calvo, Ezequiel; Soulet, Denis; Joly-Beauparlant, Charles; Droit, Arnaud; Cyr, Daniel G. ORCID logoORCID: https://orcid.org/0000-0002-6566-783X et Belleannée, Clémence (2020). Hedgehog signaling pathway regulates gene expression profile of epididymal principal cells through the primary cilium Faseb Journal , vol. 34 , nº 6. pp. 7593-7609. DOI: 10.1096/fj.202000328R.

Ce document n'est pas hébergé sur EspaceINRS.

Résumé

Primary cilia (PC) are organelles that sense and respond to dynamic changes of the extracellular milieu through the regulation of target genes. By using the epididymis as a model system, we determined the contribution of primary cilia in the regulation of epithelial cell functions through the transduction of the Hedgehog (Hh) signaling pathway. Both Sonic (SHH) and Indian Hedgehog (IHH) ligands were detected in epididymal epithelial cells by confocal microscopy and found secreted in the extracellular space. Gene expression profiling preformed on ciliated epithelial cells indicated that 153 and 1052 genes were differentially expressed following treatment with the Hh agonist SAG or the Hh antagonist cyclopamine (Cyclo), respectively. Strikingly, gene ontology analysis indicated that genes associated with immune response were the most affected following Hh modulation. The contribution of epididymal PC to canonical Hh pathway transduction was validated by ciliobrevin D treatment, which induced a significant decrease in PC length and a reduction in the expression Hh signaling targets. Such findings bring us closer to a molecular understanding of the subtle immune balance observed in some epithelia, including the epididymis and the intestine, which are organs featuring both tolerance toward autoimmune spermatozoa (or commensal bacteria) and defense against pathogens.

Type de document: Article
Mots-clés libres: epididymis; epithelial cells; immune response; inflammation; primary cilia
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 20 juill. 2021 04:00
Dernière modification: 15 févr. 2022 14:50
URI: https://espace.inrs.ca/id/eprint/11587

Gestion Actions (Identification requise)

Modifier la notice Modifier la notice