Dépôt numérique

Accelerated inactivation of M13 bacteriophage using millijoule Femtosecond Lasers

Berchtikou, Aziz, Greschner, Andrea, Tijssen, Peter, Gauthier, Marc A. et Ozaki, Tsuneyuki (2019). Accelerated inactivation of M13 bacteriophage using millijoule Femtosecond Lasers Journal of Biophotonics , vol. 13 , nº 2. p. 1-11. DOI: 10.1002/jbio.201900001.

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Irradiation of femtosecond (fs) pulse lasers in the visible and near-infrared ranges have been proposed as a promising candidate for inactivating viruses. However, in order to achieve significant virus inactivation, past works have required relatively long irradiation times (1 hour or longer), even for small volumes. Given its advantages compared with other techniques, there is an urgent need to shorten the time required to inactivate viruses using fs laser technology. In this study, we investigate the inactivation of purified M13 bacteriophage in phosphate-buffered saline with large active volume (1 cm(3) ), and short exposure time (several minutes), using lasers with 20 mJ/pulse energy at various wavelengths (800 nm, 400 nm or both 800 nm and 400 nm combined). For an exposure time of 15 min and 2 min, the use of a 400 nm wavelength laser results in a high load reduction of 5.8 +/- 0.3 and 2.9 +/- 0.15, respectively, on the log10 scale of viability. We show that virus inactivation using the 400 nm laser is much more efficient compared with that using 800 nm laser, or the simultaneous irradiation of 400 nm and 800 nm lasers. Higher pathogen inactivation is observed for lasers with shorter pulse duration, whereas at longer pulse durations, the inactivation is reduced. For millijoule-energy fs laser irradiation, the M13 bacteriophage inactivation, via the reduction of the functionality of M13 bacteriophages, is accompanied with relatively small amounts of genetic damage.

Type de document: Article
Informations complémentaires: document e201900001
Mots-clés libres: M13 bacteriophage; femtosecond laser; inactivation; load reduction
Centre: Centre Énergie Matériaux Télécommunications
Date de dépôt: 20 juill. 2021 21:43
Dernière modification: 20 juill. 2021 21:43
URI: https://espace.inrs.ca/id/eprint/11513

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