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Lipid production in Rhodosporidium toruloides using C-6 and C-5 wood hydrolysate: A comparative study.

Osorio-González, Carlos S., Hegde, Krishnamoorthy, Ferreira, Pedro, Brar, Satinder Kaur, Kermanshahipour, Azadeh, Soccol, Carlos Ricardo et Avalos-Ramírez, Antonio (2019). Lipid production in Rhodosporidium toruloides using C-6 and C-5 wood hydrolysate: A comparative study. Biomass and Bioenergy , vol. 130 . p. 105355. DOI: 10.1016/j.biombioe.2019.105355.

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Résumé

Rhodosporidium toruloides is an oleaginous yeast that can accumulate up to 70% of its dry biomass as lipids and can use C5 and C6 sugars as a carbon source, that are present in hydrolysates from different lignocellulosic biomass. R. toruloides can be an alternative for the valorization of forestry residues and the obtention of higher value-added products. In this study, five strains of R. toruloides were evaluated to find the most efficient yeast strain for C5 and C6 sugars utilization, using lignocellulosic hydrolysates as a culture media. Sugar consumption was similar between all strains. Nevertheless, it was different among C6 and C5 hydrolysates, with maximum sugar utilization of 98%, and 60% for C6 and C5 hydrolysate, respectively. Among the studied strains, the highest lipid production was observed in R. toruloides-1588 with 23.33 g of lipids/L in C6 hydrolysate after 112 h. Whereas, highest lipid production in C5 hydrolysate was observed in R. toruloides-7191 with 14.67 g of lipids/L after 120 h. Predominantly, fatty acids for R. toruloides-1588 was oleic, palmitic, and stearic acids and pentadecanoic, palmitic, and heptadecanoic acids for R. toruloides-7191. Moreover, both strains grew and produced lipids in the presence of inhibitor compounds, such as levulinic acid (13.88 mgL⁻¹), 5-HMF (80.86 mgL⁻¹), furfural (153.54 mgL⁻¹), vanillin (17.17 mgL⁻¹), vanillic acid (85.25 mgL⁻¹), syringaldehyde (41.83 mgL⁻¹), ferulic acid (1.66 mgL⁻¹), and 4-hydroxybenzoic acid (0.85 mgL⁻¹). The study revealed that R. toruloides-1588 and R. toruloides-7191 are promising strains for C5 and C6 sugar utilization from lignocellulosic hydrolysates for lipid production.

Type de document: Article
Mots-clés libres: fatty acids; lipids; Rhodosporidium toruloides; sugar utilization; lignocellulosic biomass
Centre: Centre Eau Terre Environnement
Date de dépôt: 08 mars 2021 20:44
Dernière modification: 08 mars 2021 20:44
URI: https://espace.inrs.ca/id/eprint/11380

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