Twiss, Michael R.; Errécalde, Olivier; Fortin, Claude; Campbell, Peter G. C.; Jumarie, Catherine; Denizeau, Francine; Berkelaar, Edward; Hale, Beverley et van Rees, Ken (2001). Coupling the use of computer chemical speciation models and culture techniques in laboratory investigations of trace metal toxicity. Chemical Speciation & Bioavailability , vol. 13 , nº 1. pp. 9-24. DOI: 10.3184/095422901782775462.
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The bioavailability and toxicity of a dissolved metal are closely linked to the metal’s chemical speciation in solution. A variety of inorganic and organic ligands are often used in laboratory toxicity tests to control the concentration of labile trace metal in solution. Computerised chemical speciation models based on thermodynamic principles can be used to estimate metal speciation under such experimental conditions. However, these models are sensitive to the quality of their thermodynamic databases. Detailed protocols for the incorporation of reliable equilibrium formation constants into widely available computer chemical speciation programs (e.g., MINEQL+ and MINTEQ) are provided. The examples demonstrate both the benefits and the potential pitfalls involved in the use of chemical speciation models. The application of chemical speciation modelling to metal toxicity studies is discussed and guidelines are proposed for its proper use. Both defined media and chemical speciation programs have co-existed for two decades but the combined use of these techniques has been reserved for those possessing in-depth knowledge of both chemistry and biology. The techniques presented should enable an investigator with basic biological, chemical and computing skills to design an aqueous medium and incorporate correct thermodynamic constants into a computer chemical speciation program, starting from a standardised database, thereby providing a sound framework for critically assessing the biological response of a particular test organism to a given metal.
Type de document: | Article |
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Mots-clés libres: | chemical equilibrium modelling; bioassay; methodology; experimental design |
Centre: | Centre Eau Terre Environnement |
Date de dépôt: | 11 janv. 2021 15:27 |
Dernière modification: | 11 janv. 2021 15:27 |
URI: | https://espace.inrs.ca/id/eprint/11105 |
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