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Production and in-vitro evaluation of an enzyme formulation as a potential alternative to feed antibiotics in poultry.

Suresh, Gayatri; Santos, Daniel Ubaldo; Rouissi, Tarek; Brar, Satinder Kaur; Mehdi, Youcef; Godbout, Stéphane; Chorfi, Younes; Ramirez, Antonio Avalos (2019). Production and in-vitro evaluation of an enzyme formulation as a potential alternative to feed antibiotics in poultry. Process Biochemistry , vol. 80 . p. 9-16. DOI: 10.1016/j.procbio.2019.01.023.

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Résumé

Xylanase and amylase were produced by Aspergillus niger by solid state fermentation of apple pomace. The enzyme formulation was produced by spray drying, for which, Response surface methodology was used to optimize inlet temperature (Tinlet), atomization air pressure (P) and sample feed rate (Fᵣ). Optimal conditions obtained for spray drying were Tinlet 120 °C, P 0.07 MPa, and Fr 35 ml/min. The optimized spray dried powder contained 75.3 units/g xylanase, 40.3 units/g amylase, and the humidity and outlet temperature were 5.7% and 55 °C, respectively. Poultry feed for each growth phase was supplemented with enzyme formulation (500 U/kg xylanase and 267 U/kg amylase) and subjected to digestion in an in-vitro, poultry gut model, following which, the total reducing sugar concentration (RSC) of the digesta was measured. For the in vitro digestion, an increase of 23.4%, 14.70% and 116.9% in the RSC was observed in the ileal phase for the starter, grower and finisher phase, respectively. The enzyme supplemented feeds gave a higher RSC, as compared to the control, indicating the potential capacity of the formulation to improve nutrient availability, and therefore possibly broiler performance in vitro.

Type de document: Article
Mots-clés libres: feed enzymes; apple pomace fermentation; spray drying; response surface methodology; poultry gut simulation; Aspergillus niger;
Centre: Centre Eau Terre Environnement
Date de dépôt: 03 juin 2019 18:14
Dernière modification: 03 juin 2019 18:14
URI: http://espace.inrs.ca/id/eprint/8072

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