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Fabrication of nanobiocatalyst using encapsulated laccase onto chitosan-nanobiochar composite.

Naghdi, Mitra; Taheran, Mehrdad; Brar, Satinder Kaur; Kermanshahi-pour, Azadeh; Verma, Mausam P.; Surampalli, Rao Y. (2019). Fabrication of nanobiocatalyst using encapsulated laccase onto chitosan-nanobiochar composite. International Journal of Biological Macromolecules , vol. 124 . p. 530-536. DOI: 10.1016/j.ijbiomac.2018.11.234.

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Résumé

Laccase is one of the widely used enzymes for biotechnological processes. Immobilization of enzymes is a universally accepted approach to increase their reusability and stability. In this study, laccase enzyme from Trametes versicolor was encapsulated for the first time in a chitosan-nanobiochar matrix. The chitosan-tripolyphosphate gel formation technique was employed to produce homogeneous biocatalyst nanoparticles, with 35% effective binding efficiency and 3.5 Units/g apparent activity under the best configuration. The reusability of the encapsulated laccase was demonstrated towards the oxidation of 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) for several consecutive cycles, exhibiting 30% of the initial activity after 5 cycles. The encapsulated laccase showed a moderate increase in enzyme stability against pH and temperature variation compared to the free enzyme. Moreover, the storage stability of laccase at both 4 °C and 25 °C was increased after immobilization. Only 2% of laccase was leaked during a 5-day period from biocatalyst. Laccase in its free form showed no antibacterial activity against Gram positive and Gram-negative model microorganisms, while encapsulated laccase showed antibacterial activity towards Gram-positive ones. Thus, the encapsulation of the laccase is an efficient method to keep the enzyme active and stable for different applications.

Type de document: Article
Mots-clés libres: laccase; chitosan; nanoencapsulation
Centre: Centre Eau Terre Environnement
Date de dépôt: 04 mars 2019 16:28
Dernière modification: 04 mars 2019 16:28
URI: http://espace.inrs.ca/id/eprint/7846

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