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Hair Follicle miRNAs: a Novel Biomarker for Primary Blast Induced-Mild Traumatic Brain Injury.

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Zhang, Jing; Knight, Rosalinda; Wang, Yushan; Sawyer, Thomas W.; Martyniuk, Christopher J.; Langlois, Valérie S. (2019). Hair Follicle miRNAs: a Novel Biomarker for Primary Blast Induced-Mild Traumatic Brain Injury. Biomarkers , vol. 24 , nº 2. p. 166-179. DOI: 10.1080/1354750X.2018.1531929.

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Résumé

Context: Due to the wide use of improvised explosive devices during modern warfare, primary blast-derived mild traumatic brain injury (mTBI) has become a major medical condition in the military. With minimal visually identifiable symptoms, an effective molecular biomarker system is desirable.

Objective: We assessed the potential of mammalian hair follicle miRNAs as an mTBI biomarker. Materials and methods: Due to their well-established roles in mTBI molecular pathology, the expression level of miR-183, miR-26a, miR-181c, miR-29a, miR-34a, and miR-27b was determined using qRT-PCR in whisker hair follicles from rats subject to head-only exposure to a single pulse shock wave. Based on established transcriptomics profiles, sub-network enrichment analysis (SNEA) was also conducted.

Results: The results reveled that molecular networks involving miR-183, miR-26a, and miR-181c were enriched in multiple treatments whereas sub-networks of miR-29a, miR-34a, and miR-27b were unique to individual exposure groups.

Discussion: Our study showed that all six miRNAs were reflective of the mTBI signature involved in cellular responses. Noteworthy was that the decrease in the transcript levels of miR-181c was correlated to shockwave exposure severity.

Conclusion: This study demonstrates for the first time that mammalian hair follicles are capable of housing miRNA biomarkers for TBI.

Type de document: Article
Mots-clés libres: mTBI; sub-network enrichment analysis; gene set enrichment analysis; transcriptomics; head trauma
Centre: Centre Eau Terre Environnement
Date de dépôt: 18 oct. 2018 19:23
Dernière modification: 04 oct. 2019 04:00
URI: http://espace.inrs.ca/id/eprint/7685

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