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Phthalates modulate steroid 5-reductase transcripts in the Western clawed frog embryo.

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Bissegger, Sonja; Pineda Castro, Marco A.; Yargeau, Viviane; Langlois, Valérie S. (2018). Phthalates modulate steroid 5-reductase transcripts in the Western clawed frog embryo. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology , vol. 213 . p. 39-46. DOI: 10.1016/j.cbpc.2018.07.005.

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Résumé

Phthalates are used worldwide in the manufacturing of plastics, added to cosmetic products, personal care products, pharmaceuticals, medical devices, and paints; and are widely detected in soil, surface water, and organism tissues. Phthalate esters have been previously shown to interfere with the endocrine system in vertebrates. However, few studies have investigated the effects of phthalates on testosterone-converting enzymes that affect hormone levels and reproduction. In the present study, we exposed the Western clawed frog (Silurana tropicalis) to 0.1, 1, and 10 μM diethylhexyl phthalate (DEHP), dibutyl phthalate (DBP), and diethyl phthalate (DEP) during early amphibian embryonic development. Additional DBP exposures were conducted ex vivo using mature frog testes. Malformations and mRNA levels of genes associated to reproduction and oxidative stress were evaluated. 0.1 μM DEHP, DBP, and DEP induced an array of malformations, including incomplete gut coiling, edemas, and eye malformations. Moreover, all three phthalates increased the expression of androgen-related genes, such as steroid-5α-reductase 1, 2, 3, steroid-5β-reductase, and androgen receptor at concentrations ranging from 0.1 to 10 μM depending on the phthalate and gene. Data suggest that the phthalate esters tested are teratogens to the amphibian embryo and that these phthalates exhibit an androgenic activity in amphibians.

Type de document: Article
Mots-clés libres: androgen disruption; DHEP; DBP; DEP; Srd5α1; Srd5α2; Srd5α3; Srd5β
Centre: Centre Eau Terre Environnement
Date de dépôt: 18 oct. 2018 18:50
Dernière modification: 26 juill. 2019 04:00
URI: http://espace.inrs.ca/id/eprint/7667

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