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Herpes simplex virus 1 infection of T cells causes VP11/12-dependent phosphorylation and degradation of the cellular protein Dok-2

Lahmidi, Soumia; Strunk, Ulrike; Smiley, James R.; Pearson, Angela; Duplay, Pascale (2017). Herpes simplex virus 1 infection of T cells causes VP11/12-dependent phosphorylation and degradation of the cellular protein Dok-2 Virology , vol. 511 . p. 66-73. DOI: 10.1016/j.virol.2017.08.018.

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Résumé

Previous studies have shown that HSV-1 infection of lymphocytes induces the tyrosine phosphorylation of several proteins that might correspond to viral or host proteins. VP11/12, a viral tegument protein, is the major HSV-induced tyrosine phosphorylated protein identified thus far. In this report, we demonstrated that the cellular adaptor proteins Dok-2 and Dok-1 are tyrosine phosphorylated upon HSV-1 infection. In addition, HSV-1 induced the selective degradation of Dok-2. Finally, we provide evidence that Dok-2 interacts with VP11/12, and that HSV-induced tyrosine phosphorylation and degradation of Dok-2 require VP11/12. Inactivation of either the Src Family Kinases binding motifs or the SHC binding motif of VP11/12 eliminated the interaction of Dok-2 with VP11/12. Elimination of the binding of Dok-2 to VP11/12 prevented Dok-2 phosphorylation and degradation. We propose that HSV-induced Dok phosphorylation and Dok-2 degradation is an immune evasion mechanism to inactivate T cells that might play an important role in HSV pathogenesis.

Type de document: Article
Mots-clés libres: Dok, Herpes simplex virus, VP11/12, Tegument, T cell, Immune evasion
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 10 mars 2019 19:27
Dernière modification: 10 mars 2019 19:27
URI: http://espace.inrs.ca/id/eprint/7527

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