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T lymphocyte-proliferative responses of harbor seal (Phoca vitulina) peripheral blood mononuclear cells (PBMCs) exposed to pharmaceuticals in vitro

Kleinert, Christine; Lacaze, Emilie; Fortier, Marlène; Hammil, Mike; De Guise, Sylvain; Fournier, Michel (2018). T lymphocyte-proliferative responses of harbor seal (Phoca vitulina) peripheral blood mononuclear cells (PBMCs) exposed to pharmaceuticals in vitro Marine Pollution Bulletin , vol. 127 . p. 225-234. DOI: 10.1016/j.marpolbul.2017.12.001.

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Résumé

The ubiquity of pharmaceuticals in the aquatic environment and the accumulation in organisms of lower trophic levels have been documented. The immunotoxicity of these xenobiotics has however been little investigated. This study assessed the effects of pharmaceuticals on the immune responses of harbor seal lymphocytes. Peripheral blood mononuclear cells isolated from harbor seal pups were exposed to varying concentrations of 17α-ethinyl estradiol (250-50,000μg/L), naproxen (500-100,000μg/L), carbamazepine (500-100,000μg/L), erythromycin (750-150,000μg/L) and binary mixtures thereof in vitro. All individual compounds and mixtures inhibited lymphocyte proliferation. Mixture effects were non-additive and predictive values overestimated the inhibition of proliferation. Male pups were more sensitive to erythromycin exposure. Comparison with the sensitivity of the 11B7501 cell line showed a higher sensitivity of pups to individual compounds and the inverse trend for mixtures. Based on our results, we hypothesize that pharmaceuticals may have the potential to interrupt immune functions in harbor seals.

Type de document: Article
Mots-clés libres: Immunotoxicity; Marine mammal; Pharmaceuticals; Phoca vitulina
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 04 mars 2019 18:10
Dernière modification: 04 mars 2019 18:10
URI: http://espace.inrs.ca/id/eprint/7462

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