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Role of the protein SHP-1 during the process of phagocytosis in murine macrophages

Gomez, Carolina P; Shio, Marina Tiemi; Olivier, Martin; Descoteaux, Albert . Role of the protein SHP-1 during the process of phagocytosis in murine macrophages In: 10e Symposium Annuel de Parasitologie Moléculaire du Québec, 8-9 juin 2010, Université McGill.

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Résumé

The process of phagocytosis and phagosomal maturation involves the recruitment of several proteins that participate in the formation of phagosome, and in the acidification and/or fusion with other vesicules in order to either recycle cellular compounds or degrade cellular debris and parasites. Among these proteins, phosphatases are known to be involved in such processes (p. ex: SHIP-1), and in some cases, are used by the intracellular parasites to delay the maturation process to their advantage (p.e: M. tuberculosis). However, the role of the protein SHP-1 in such process has yet to be determined. Using murine macrophage cell lines defficient for SHP-1 (motheaten [Me SHP-1-/-]), and their wild type littermate we studied the kinetics of the recruitment of the Lysosomal-associated membrane protein 1 (LAMP1), a known marker for phagosomal maturation. Using confocal microscopy and Western Blot we noticed a delayed recruitment in SHP-1-/- cells. Also, we noticed that the active form of Cathepsin D (Cath D), which is also related to the maturation process in phagosomes, is presented earlier in the phagosome isolation extracts from SHP-1+/+ cells, and this is not due to an absence of the precursor in the cells. Furthermore, there seems to be a higher recruitment of the precursor in the SHP-1-/- cells.

Type de document: Document issu d'une conférence ou d'un atelier
Informations complémentaires: Présentation par affiche
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Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 02 mai 2018 21:21
Dernière modification: 02 mai 2018 21:21
URI: http://espace.inrs.ca/id/eprint/7107

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