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Leishmania promastigotes induce and are killed by Neutrophil Extracellular Traps

Gabriel, Christelle; Girard, Denis; Descoteaux, Albert . Leishmania promastigotes induce and are killed by Neutrophil Extracellular Traps In: 9e symposium annuel de parasitologie moléculaire du Québec, 18-19 juin 2009, Université McGill.

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Résumé

The role of neutrophils during early stage of infection by Leishmania is not clear. Due to their rapid recruitment to the site of inoculation, neutrophils are the first immune cells to interact with the parasite. Recent evidence suggest that neutrophils can phagocyte promastigotes which interestingly survive into cells during few days. Initially, we investigated how promastigotes resist human neutrophil oxidative killing. Unexpectedly, we found that in contact to Leishmania promastigotes, neutrophils released structures which seemed to be Neutrophil Extracellular Traps (NETs). Production of NETs is a recent discovered microbicidal mechanism borrowed by neutrophils and is characterized by the release of DNA and some granular proteins from stimulated-neutrophils. This study was conducted to determine whether Leishmania promastigotes induce the production of NETs by human neutrophils and the impact of those structures on the parasite. Using confocal microscopy and DNA quantification we observed that independently strains and species, Leishmania quickly induced in a ratio dependant-manner the formation of NETs. Using defective promastigotes on lipophosphoglycan, we also observed that this important virulence factor was not involved in this process. Then, quantification of the promastigotes survival following contact with neutrophils showed an efficient killing of Leishmania. Thus, our results strongly describe NETs as a novel host response against Leishmania. This work was supported by the Canadian Institutes of Health Research and the Canada Research Chair Program.

Type de document: Document issu d'une conférence ou d'un atelier
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Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 02 mai 2018 20:14
Dernière modification: 02 mai 2018 20:14
URI: http://espace.inrs.ca/id/eprint/7103

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