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Impact of Leishmania donovani phosphoglycans on the macrophage transcriptome

Gabriel, Christelle; Dogra, Nisha; McMaster, W. Robert; Descoteaux, Albert . Impact of Leishmania donovani phosphoglycans on the macrophage transcriptome In: 8e symposium annuel de parasitologie moléculaire du Québec, 12-13 juin 2008, UQAM.

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Résumé

During its life cycle, the biphasic organism Leishmania donovani infects macrophages. Depending on the parasite species and developmental stage as well as on the activation stage of its host cell, L. donovani can prevent or activate cellular signalling pathway and the resulting cellular responses. The promastigote of L. donovani is coated by a glycocalyx composed of glycoconjugates containing the repeating unit Galb14Man-PO4. Previous studies have shown that lipophosphoglycan (LPG), the major surface glycoconjugate of Leishmania donovani promastigotes, plays a critical role for the macrophage infection. Indeed, LPG strongly modulate expression of specific host cell genes and proteins. Nevertheless, little is known about the global effect of L. donovani glycoconjugates on macrophage gene expression. In this study, we therefore used microarrays to compare genetic expression profiles of macrophages infected with wild-type promastigotes or mutants (lpg2-KO) defective for the synthesis of glycoconjugates containing repeating unit of Galb14Man-PO4. Infected macrophages were either naive or previously stimulated with IFN-γ. After RNA extraction at different times post-infection and transformation into cDNA, samples were analysed by Affymetrix GeneChip Expression Arrays. The analysis shows that the majority of regulated genes by the glyconcojugates are implicated in critical functions such as signal transduction, transcription and metabolism. To validate the results of the arrays, quantitative PCR for some genes are currently under way.

Type de document: Document issu d'une conférence ou d'un atelier
Informations complémentaires: Présentation par affiche
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Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 02 mai 2018 20:07
Dernière modification: 02 mai 2018 20:08
URI: http://espace.inrs.ca/id/eprint/7102

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