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Translational control of placental cells during Toxoplasma gondii infection

Chaparro, Visnu; Leroux, Louis-Phillipe; Raisch, Jennifer; Saljoughian, Noushin; Vaillancourt, Cathy; Jaramillo, Maritza . Translational control of placental cells during Toxoplasma gondii infection In: 16e symposium annuel de parasitologie moléculaire du Québec, 6-7 juin 2016, Université McGill, Montréal, QC.

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Résumé

Toxoplasma gondii is one of the most successful parasites in the world. In humans, it can be transmitted vertically to the fetus through the placenta, where it can cause blindness, hydrocephalus, mental retardation and even miscarriage. The placenta is a mixed tissue formed by the maternal endometrium and the fetal trophoblast, being the latter considered the point of entry of the parasite to the fetal cavity. Since no prophylactic immunotherapies or safe treatments during pregnancy are available, it is of utter importance to understand the interaction of the parasite with its host cells in order to develop more effective control strategies. In this context, we are investigating the role of translational control exerted by T. gondii over infected trophoblasts using the choriocarninoma BeWo cell line. To this end, we evaluate the modulation of the MAPK and mTORC1 pathways, which regulate the translation initiation-related proteins eIF4E, 4E-BP1/2 and S6. We observed that upon T. gondii infection, the rate of protein synthesis increases dramatically. This correlates with an activation of the mTORC1 pathway, as evidenced by the phosphorylation of its downstream targets S6 and 4E-BP1. In contrast, the MAPK pathway does not seem to be affected when we assessed the phosphorylation of their downstream targets Mnk1/2 and eIF4E. Overall, our results suggest that upon T. gondii infection there is a change on the global rate of translation. Whether this is a strategy of parasite survival or a mechanism of host defense, or a combination of both is a question that remains to be addressed. Currently, we are further characterizing the molecular mechanisms that mediate this event and identifying the mRNAs that are differentially modulated.

Type de document: Document issu d'une conférence ou d'un atelier
Informations complémentaires: Présentation par affiche
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Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 01 mai 2018 20:40
Dernière modification: 01 mai 2018 20:40
URI: http://espace.inrs.ca/id/eprint/7078

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