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Moesin and myosin IIA modulate phagolysosomal biogenesis in macrophages

Gomez, Carolina P; Descoteaux, Albert (2018). Moesin and myosin IIA modulate phagolysosomal biogenesis in macrophages Biochemical and Biophysical Research Communications , vol. 495 , nº 2. p. 1964-1971. DOI: 10.1016/j.bbrc.2017.12.061.

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Résumé

Biogenesis of phagolysosomes is central to the elimination of pathogens by macrophages. We previously showed that Src homology region 2 domain-containing phosphatase 1 (SHP-1) participates in the regulation of phagosome maturation. Through proteomics, we identified moesin and the non-muscle myosin-IIA as proteins interacting with SHP-1 during phagocytosis. Silencing of either moesin or myosin IIA with small interfering RNA inhibited phagosomal acidification and recruitment of LAMP-1. Moreover, the intraphagosomal oxidative burst was impaired in the absence of either SHP-1 or myosin IIA but not moesin. Finally, absence of either SHP-1, moesin, or myosin IIA ablated the capacity of macrophages to clear bacterial infection. Collectively, these results implicate both moesin and myosin IIA in the regulation of phagolysosome biogenesis and in host defense against infections. © 2017 Elsevier Inc.

Type de document: Article
Mots-clés libres: SHP-1; Moesin; Myosin IIA; Phagolysosome biogenesis; Macrophage
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 25 mars 2019 18:44
Dernière modification: 25 mars 2019 18:44
URI: http://espace.inrs.ca/id/eprint/6728

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