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Covalent Immobilization of laccase onto nanofibrous membrane for degradation of pharmaceutical residues in water.

Taheran, Mehrdad; Naghdi, Mitra; Brar, Satinder Kaur; Knystautas, Emile J; Verma, Mausam; Surampalli, Rao Y. (2017). Covalent Immobilization of laccase onto nanofibrous membrane for degradation of pharmaceutical residues in water. ACS Sustainable Chemistry & Engineering . DOI: 10.1021/acssuschemeng.7b02465.

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Résumé

Enzymatic degradation with ligninolytic enzyme e.g. laccase is a potential green solution for removal of pharmaceutical compounds that are released into the environment through wastewater effluent. However, the deficiencies of using the enzyme in its free forms, such as reusability and stability should be addressed before industrial applications. In this study, laccase was immobilized onto tailor-made Polyacrylonitrile-biochar composite nanofibrous membrane through covalent bonding and the parameters of immobilization were optimized. The obtained biocatalyst was utilized for removal of chlortetracycline (CTC), carbamazepine (CBZ) and diclofenac (DCF) at an environmentally-relevant concentration in batch mode. These pharmaceutical compounds represented three main categories of pharmaceutical compounds i.e. antibiotics, antidepressant, and anti-inflammatory. The results showed that the immobilized laccase has improved storage, temperature and pH stability compared to free laccase. Also, it maintained more than 17 % of its initial activity after 10 cycles of ABTS oxidation which indicated improved reusability of the enzyme. Using immobilized laccase for degradation of three pharmaceutical compounds in batch experiments exhibited 72.7%, 63.3% and 48.6% degradation efficiency for DCF, CTC, and CBZ, respectively after 8 hours of reaction. The decreasing trend of adsorption extent during reaction time for all compounds confirmed the regenerative effect of laccase on adsorption sites of biochar.

Type de document: Article
Mots-clés libres: Biodegradation; Laccase; Pharmaceutical compounds; Wastewater
Centre: Centre Eau Terre Environnement
Date de dépôt: 27 nov. 2017 21:57
Dernière modification: 06 déc. 2017 21:18
URI: http://espace.inrs.ca/id/eprint/6375

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