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Survey of High-Affinity H2-Oxidizing Bacteria in Soil Reveals Their Vast Diversity Yet Underrepresentation in Genomic Databases

Piche-Choquette, Sarah; Khdhiri, Mondher; Constant, Philippe (2017). Survey of High-Affinity H2-Oxidizing Bacteria in Soil Reveals Their Vast Diversity Yet Underrepresentation in Genomic Databases Microbial Ecology , vol. 74 , nº 4. p. 771-775. DOI: 10.1007/s00248-017-1011-1.

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Résumé

While high-affinity H2-oxidizing bacteria (HA-HOB) serve as the main sink of atmospheric H2, the ecology of this specialist functional group is rather unknown due to its recent discovery. The main purpose of our study is to provide the first extensive survey of HA-HOB in farmland, larch, and poplar soils exposed to 0.5 and 10,000 ppmv H2. Using qPCR and qRT-PCR assays along with PCR amplicon high-throughput sequencing of hhyL gene encoding for the large subunit of high-affinity [NiFe]-hydrogenases (HAH), we found that hhyL gene expression ratio explained better variation in measured H2 oxidation rates than HA-HOB species richness. Carbon, nitrogen, pH, and bacterial species richness appeared as the most important drivers of HA-HOB community structure. Our study also highlights the need to cultivate HA-HOB due to the huge gap in current genomic databases.

Type de document: Article
Mots-clés libres: Biogeochemistry; H2-oxidizing bacteria; High-throughput sequencing; Trace gas
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 27 févr. 2019 20:56
Dernière modification: 06 mars 2019 21:17
URI: http://espace.inrs.ca/id/eprint/6265

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