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Elucidating the Effect of Glycerol Concentration and C/N Ratio on Lipid Production Using Yarrowia lipolytica SKY7.

Kuttiraja, Mathiazhakan; Ayed, Dhouha; Valéro, José R.; Tyagi, Rajeshwar Dayal (2016). Elucidating the Effect of Glycerol Concentration and C/N Ratio on Lipid Production Using Yarrowia lipolytica SKY7. Applied Biochemistry and Biotechnology , vol. 180 , nº 8. p. 1586-1600. DOI: 10.1007/s12010-016-2189-2.

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Résumé

The high demand for renewable energy and increased biodiesel production lead to the surplus availability of crude glycerol. Due to the above reason, the bio-based value addition of crude glycerol into various bioproducts is investigated; among them, microbial lipids are attractive. The present study was dedicated to find the optimal glycerol concentration and carbon/nitrogen (C/N) ratio to produce maximum lipid using Yarrowia lipolytica SKY7. The glycerol concentration (34.4 to168.2 g/L) and C/N ratio (25 to 150) were selected to investigate to maximize the lipid production. Initial glycerol concentration 112.5 g/L, C/N molar ratio of 100, and with 5 % v/v inoculum supplementation were found to be optimum for biomass and lipid production. Based on the above optimal parameters, lipid concentration of 43.8 % w/wwith a biomass concentration of 14.8 g/L was achieved. In the case of glycerol concentration, the maximum Yp/s (0.192 g/g); Yx/s (0.43 g/g) was noted when the initial glycerol concentration was 112.5 g/L with C/N molar ratio 100 and inoculum volume 5 % v/v. The glycerol uptake was also noted to increase with the increase in glycerol concentration. At low C/N ratio, the glycerol consumption was found to be high (79.43 g/L on C/N 25) whereas the glycerol consumption was observed to decrease when the C/N ratio was raised to 150 (40.8 g/L).

Type de document: Article
Mots-clés libres: Y. lipolytica; crude glicerol; biodiesel; microbial lipid; C/N ratio
Centre: Centre Eau Terre Environnement
Date de dépôt: 03 mai 2018 15:18
Dernière modification: 03 mai 2018 15:18
URI: http://espace.inrs.ca/id/eprint/5699

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