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Pilot-scale biopesticide production by Bacillus thuringiensis subsp. kurstaki using starch industry wastewater as raw material

Ndao, Adama; Balasubramanian, Sellamuthu; Gnepe, Jean Robert; Tyagi, Rajeshwar Dayal; Valéro, José R. (2017). Pilot-scale biopesticide production by Bacillus thuringiensis subsp. kurstaki using starch industry wastewater as raw material Journal of Environmental Science and Health, Part B Pesticides, Food Contaminants, and Agricultural Wastes , vol. 52 , nº 9. p. 623-630. DOI: 10.1080/03601234.2017.1330071.

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Résumé

Pilot-scale Bacillus thuringiensis based biopesticide production (2000 L bioreactor) was conducted using starch industry wastewater (SIW) as a raw material using optimized operational parameters obtained in 15 L and 150 L fermenters. In pilot scale fermentation process the oxygen transfer rate is a major limiting factor for high product yield. Thus, the volumetric mass transfer coefficient (KLa) remains a tool to determine the oxygen transfer capacity [oxygen utilization rate (OUR) and oxygen transfer rate (OTR)] to obtain better bacterial growth rate and entomotoxicity in new bioreactor process optimization and scale-up. This study results demonstrated that the oxygen transfer rate in 2000 L bioreactor was better than 15 L and 150 L fermenters. The better oxygen transfer in 2000 L bioreactor augmented the bacterial growth [total cell (TC) and viable spore count (SC)] and delta-endotoxin yield. Prepared a stable biopesticide formulation for field use and its entomotoxicity was also evaluated. This study result corroborates the feasibility of industrial scale operation of biopesticide production using starch industry wastewater as raw material.

Type de document: Article
Mots-clés libres: Bacillus thuringiensis; Biopesticide formulation; KLa; bioreactor; scale-up; starch industry wastewater
Centre: Centre Eau Terre Environnement
Centre INRS-Institut Armand Frappier
Date de dépôt: 23 déc. 2017 14:06
Dernière modification: 23 déc. 2017 14:07
URI: http://espace.inrs.ca/id/eprint/5564

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