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Immunotoxic effects of single and combined pharmaceuticals exposure on a harbor seal (Phoca vitulina) B lymphoma cell line

Kleinert, Christine; Lacaze, Emilie; Mounier, Meryl; De Guise, Sylvain; Fournier, Michel (2017). Immunotoxic effects of single and combined pharmaceuticals exposure on a harbor seal (Phoca vitulina) B lymphoma cell line Marine Pollution Bulletin , vol. 118 , nº 1-2. p. 237-247. DOI: 10.1016/j.marpolbul.2017.02.041.

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Résumé

The potential risk of pharmaceuticals in the environment to top-predators is still largely unknown. In this study, we assessed the immunotoxic effects of ten pharmaceuticals individually and as mixtures on a harbor seal (Phoca vitulina) B lymphoma cell line. A significant reduction in lymphocyte transformation was observed following an exposure to 12,500mug/L 17alpha-ethinyl estradiol and 25,000mug/L naproxen. Exposure to 12,500mug/L 17alpha-ethinyl estradiol decreased the percentage of cell in the G0/G1 phase of the cell cycle while increasing the percentage of cells in the S phase. Carbamazepine exposure increased the amount of cells in the G2/M phase. Binary mixtures showed synergistic effects in lymphocyte transformation, cell cycle and apoptosis assays. Concentrations inducing toxic effects in the cell line were similar to those affecting fish in previous studies. A reduction of functional activities of the immune system may lead to altered host resistance to pathogens in free-ranging pinnipeds.

Type de document:
Mots-clés libres: Cell line; Harbor seal; Immunotoxicity; In vitro; Mixture; Pharmaceuticals
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 05 juill. 2017 20:57
Dernière modification: 05 juill. 2017 20:57
URI: http://espace.inrs.ca/id/eprint/5384

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