Dépôt numérique
RECHERCHER

Double resonance long period fiber grating for detection of E. coli in trace concentration by choosing a proper bacteriophage

Chiniforooshan, Yasser; Celebanska, Anna; Janik, Monika; Mikulic, Predrag; Haddad, Fayrouz; Perreault, Jonathan; Bock, Wojtek J. (2017). Double resonance long period fiber grating for detection of E. coli in trace concentration by choosing a proper bacteriophage In: 25th International Conference on Optical Fiber Sensors, April, 24-28 2017, Jeju, South Korea.

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Résumé

There is a critical need of a fast, specific and reliable assay for biological species. To address this need, long period fiber gratings (LPFG) among other fiber optic sensors can be used because of their high sensitivity to changes in surrounding medium. In this work we fabricated and used two over-etched LPFGs. One of them was covered with T4 Phage and the other was covered with MS2 phage that both specifically bind with Escherichia coli (E. coli) bacteria. This bacterium is a major cause of the food contaminations and outbreaks. We showed achieving a highest sensitivity region of the LPFG and the way to fine tune to that region by over-etching the grating. Finally, using the highly sensitive LPFG platform we could detect E. coli at concentrations as low as 100 colony forming units (CFU), by covering the LPFG with an optimized bio-functionalization of the fiber surface with MS2 bacteriophage.

Type de document: Document issu d'une conférence ou d'un atelier
Informations complémentaires: Proceedings of SPIE - Volume 10323 - Poster Session http://dx.doi.org/10.1117/12.2265950
Mots-clés libres: Biosensor; Double resonance; E. coli bacteria; Long period fiber grating LPFG; MS2 bacteriophage; Over etched LPFG; T4 bacteriophage; Trace detection; Bacteria; Bacteriophages; Biosensors; Escherichia coli; Fiber optic sensors; Fibers; Optical fiber fabrication; Optical fibers; Trace analysis; E. coli; Long period fiber grating; T4 bacteriophages; Diffraction gratings
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 26 févr. 2019 20:16
Dernière modification: 26 févr. 2019 20:16
URI: http://espace.inrs.ca/id/eprint/5335

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