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Detergent assisted lipid extraction from wet yeast biomass for biodiesel: A response surface methodology approach.

Yellapu, Sravan Kumar; Bezawada, Jyothi; Kaur, Rajwinder; Kuttiraja, Mathiazhakan; Tyagi, Rajeshwar Dayal (2016). Detergent assisted lipid extraction from wet yeast biomass for biodiesel: A response surface methodology approach. Bioresource Technology , vol. 218 . p. 667-673. DOI: 10.1016/j.biortech.2016.07.011.

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Résumé

The lipid extraction from the microbial biomass is a tedious and high cost dependent process. In the present study, detergent assisted lipids extraction from the culture of the yeast Yarrowia lipolytica SKY-7 was carried out. Response surface methodology (RSM) was used to investigate the effect of three principle parameters (N-LS concentration, time and temperature) on microbial lipid extraction efficiency % (w/w). The results obtained by statistical analysis showed that the quadratic model fits in all cases. Maximum lipid recovery of 95.3 ± 0.3% w/w was obtained at the optimum level of process variables [N-LS concentration 24.42 mg (equal to 48 mg N-LS/g dry biomass), treatment time 8.8 min and reaction temperature 30.2 °C]. Whereas the conventional chloroform and methanol extraction to achieve total lipid recovery required 12 h at 60 °C. The study confirmed that oleaginous yeast biomass treatment with N-lauroyl sarcosine would be a promising approach for industrial scale microbial lipid recovery.

Type de document: Article
Mots-clés libres: lipid extraction; N-lauroyl sarcosine; Box-Behnken design; biodiesel
Centre: Centre Eau Terre Environnement
Date de dépôt: 03 mai 2018 18:35
Dernière modification: 03 mai 2018 18:35
URI: http://espace.inrs.ca/id/eprint/4712

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