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Optimization and Production of Silver-Protein Conjugate as Growth Inhibitor.

Bhattacharya, Indrani; Bezawada, Jyothi; Yan, Song; Tyagi, Rajeshwar Dayal (2015). Optimization and Production of Silver-Protein Conjugate as Growth Inhibitor. Journal of Bionanoscience , vol. 9 , nº 4. p. 261-269. DOI: 10.1166/jbns.2015.1309.

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Résumé

Williopsis saturnus is known as the effective killer yeast against non-candida albicans spp. Efficiency of W. saturnus can be increased if the killer protein is produced in nanometer size. The capacity of W. saturnus killer protein KT4561 for the production of silver nanoparticles has been explored for the first time. Therefore, synthesis of silver nanoparticles (Ag-NPs) by optimization of various parameters and their characterization has been carried out in this study. It was found that Ag-NPs were completely synthesized using 4.3 g/L W. saturnus protein and 200 μL of 0.1 M AgNO₃ (3.96 mM) at reaction time of 4 h. FTIR results confirms that only protein bio-molecules are bonded with reduced silver for synthesis of NPs. It is also observed from EDS results that silver is the only metal which is responsible for the formation of NPs. The size of synthesized NPs is approximately 200 nm. SEM results revealed that synthesized NPs are formed as crystalline structures. The killer activity of the Ag-KT4561 (silver nanoparticles) has increased 3-folds compared to KT4561 (pure protein) which was observed during well-diffusion method. Bulk production of Ag-KT4561 is feasible, therefore, can be used for growth inhibition as it is economical, time-saving, and also supports green synthesis.

Type de document: Article
Mots-clés libres: green chemistry; growth inhibition; killer protein (kt4561); reaction time; silver nanoparticles (ag-nps); W. saturnus
Centre: Centre Eau Terre Environnement
Date de dépôt: 02 mai 2018 19:42
Dernière modification: 02 mai 2018 19:42
URI: http://espace.inrs.ca/id/eprint/4677

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