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Effects of Toll-like receptor ligands on RAW 264.7 macrophage morphology and zymosan phagocytosis

Sigola, Lynette B.; Fuentes, Ana-Lucia; Millis, Leonard M.; Vapenik, Jacqueline; Murira, Armstrong (2016). Effects of Toll-like receptor ligands on RAW 264.7 macrophage morphology and zymosan phagocytosis Tissue & Cell , vol. 48 , nº 4. p. 389-396. DOI: 10.1016/j.tice.2016.04.002.

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Résumé

In this study we compared the effects of the Toll-like receptor (TLR) ligands lipopolysaccharide (LPS), flagellin, the synthetic bacterial triacylated lipopeptide Pam3-Cys-Ser-Lys4 (Pam(3)CSK(4)), Polyinosinic:polycytidylic acid (Poly I:C), and macrophage-activating lipopeptide (MALP-2), which are TLR4, TLR5, TLR1/2, TLR3, and TLR2/6 agonists, respectively, on cell morphology and phagocytosis of zymosan particles, derived from Saccharomyces cerevisiae, and rich in fungal PAMPs including beta-glucan, man nose, and chitin. LPS, Pam(3) CSK4, and MALP-2 induced an activated macrophage phenotype and enhanced zymosan phagocytosis. In contrast, flagellin and Poly I:C, respectively, had little effect on cell morphology and phagocytosis. We examined the role of scavenger receptor A (SR-A) on zymosan phagocytosis. Cells cultured in medium alone expressed SR-A, and LPS induced further expression of the receptor. We also observed inhibitory effects of scavenger receptor antagonists fucoidan, dextran sulphate, and Polyinosinic (Poly I), respectively, on zymosan phagocytosis of cells in medium alone and those pre-treated with LPS. We conclude that exposure to specific TLR ligands impacts both cellular morphology and phagocytic capacity, and that scavenger receptors contribute to zymosan ingestion as well as LPS-induced augmentation of phagocytosis. (C) 2016 Elsevier Ltd. All rights reserved.

Type de document: Article
Mots-clés libres: Macrophage; Morphology; Phagocytosis; Scavenger receptor; Toll-like receptor; Zymosan
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 06 oct. 2017 14:23
Dernière modification: 06 oct. 2017 14:23
URI: http://espace.inrs.ca/id/eprint/4587

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