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Encapsulation of ligninolytic enzymes and its application in clarification of juice.

Gassara-Chatti, Fatma; Brar, Satinder Kaur; Ajila, Chandran Matheyambath; Verma, Mausam; Tyagi, Rajeshwar Dayal; Valéro, José R. (2013). Encapsulation of ligninolytic enzymes and its application in clarification of juice. Food Chemistry , vol. 137 , nº 1-4. p. 18-24. DOI: 10.1016/j.foodchem.2012.09.083.

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Résumé

The thermal stability, physico-chemical properties and effect on juice clarification of hydrogel formulations of ligninolytic enzymes from Phanerochaete chrysosporium were evaluated. The results showed that enzyme entrapment increase significantly (P < 0.05) the thermal stability of enzymes at 4 and 75 °C. At 75 °C, maximum activity decreased to non detectable values of 7.9% for free laccase, manganese peroxidase (MnP), lignin peroxidase (LiP), respectively; to 94%, 97%, 93% for laccase, MnP and LiP entrapped into Polyacrylamide/pectin, 94%, 98%, 88% for laccase, MnP and LiP encapsulated respectively into polyacrylamide/ gelatine and to 87%, 91%, 87% for laccase, MnP and LiP entrapped, respectively into polyacrylamide/carboxymethylcellulose (CMC). When particle size and viscosity of the formulation increased, enzyme stability increased. The polyphenolic reduction and clarity amelioration in mixed juice of berry and pomegranate was more significant (p > 0.05) using encapsulated enzymes treatment than free enzymes. This suggested that enzymatic treatment was efficient for the juice clarification.

Type de document:
Mots-clés libres: enzyme encapsulation; juice clarification; ligninolytic enzymes; polyacrylamide gel
Centre: Centre Eau Terre Environnement
Date de dépôt: 05 déc. 2016 20:49
Dernière modification: 11 janv. 2017 20:34
URI: http://espace.inrs.ca/id/eprint/3437

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