Dépôt numérique
RECHERCHER

Secondary conformational conversion is involved in glycosaminoglycans-mediated cellular uptake of the cationic cell-penetrating peptide PACAP

Tchoumi Neree, Armelle; Nguyen, Phuong Trang; Chatenet, David; Fournier, Alain; Bourgault, Steve (2014). Secondary conformational conversion is involved in glycosaminoglycans-mediated cellular uptake of the cationic cell-penetrating peptide PACAP FEBS Letters , vol. 588 , nº 24. p. 4590-4596. DOI: 10.1016/j.febslet.2014.10.029.

[img]
Prévisualisation
PDF - Version acceptée
Disponible sous licence Creative Commons Attribution Non-commercial.

Télécharger (893kB) | Prévisualisation

Résumé

Glycosaminoglycans (GAGs) contribute to the cellular uptake of cationic cell-penetrating peptides (CPPs). However, molecular details about the contributions of GAGs in CPP internalization remain unclear. In this study, we examined the cellular uptake mechanism of the arginine-rich CPP pituitary adenylate-cyclase-activating polypeptide (PACAP). We observed that the uptake efficacy of PACAP is dependent on the expression of cell surface GAGs. As the binding of PACAP to sulfated GAGs induced a random coil-to-α-helix conformational conversion, we investigated the role of the helical formation in PACAP internalization. Whereas this secondary structure was not crucial for efficient internalization in GAGs-deficient cells, PACAP α-helix was essential for GAGs-dependent uptake.

Type de document:
Mots-clés libres: Cell-penetrating peptide; Cellular uptake; Glycosaminoglycan; Pituitary adenylate cyclase-activating polypeptide α-Helix
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 23 juin 2017 04:20
Dernière modification: 23 juin 2017 04:21
URI: http://espace.inrs.ca/id/eprint/3123

Actions (Identification requise)

Modifier la notice Modifier la notice