Dépôt numérique
RECHERCHER

Deficiency in Either 4E-BP1 or 4E-BP2 Augments Innate Antiviral Immune Responses

Nehdi, Atef; Sean, Polen; Linares, Izzar; Colina, Rodney; Jaramillo, Maritza; Alain, Tommy (2014). Deficiency in Either 4E-BP1 or 4E-BP2 Augments Innate Antiviral Immune Responses PLoS ONE , vol. 9 , nº 12. e114854. DOI: 10.1371/journal.pone.0114854.

[img]
Prévisualisation
PDF
Disponible sous licence Creative Commons Attribution.

Télécharger (1MB) | Prévisualisation

Résumé

Genetic deletion of both 4E-BP1 and 4E-BP2 was found to protect cells against viral infections. Here we demonstrate that the individual loss of either 4E-BP1 or 4E-BP2 in mouse embryonic fibroblasts (MEFs) is sufficient to confer viral resistance. shRNA-mediated silencing of 4E-BP1 or 4E-BP2 renders MEFs resistant to viruses, and compared to wild type cells, MEFs knockout for either 4E-BP1 or 4E-BP2 exhibit enhanced translation of Irf-7 and consequently increased innate immune response to viruses. Accordingly, the replication of vesicular stomatitis virus, encephalomyocarditis virus, influenza virus and Sindbis virus is markedly suppressed in these cells. Importantly, expression of either 4E-BP1 or 4E-BP2 in double knockout or respective single knockout cells diminishes their resistance to viral infection. Our data show that loss of 4E-BP1 or 4E-BP2 potentiates innate antiviral immunity. These results provide further evidence for translational control of innate immunity and support targeting translational effectors as an antiviral strategy.

Type de document:
Mots-clés libres: -
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 09 mars 2016 21:52
Dernière modification: 09 mars 2016 21:52
URI: http://espace.inrs.ca/id/eprint/3087

Actions (Identification requise)

Modifier la notice Modifier la notice