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Exposure of fathead minnows to municipal wastewater effluent affects intracellular signaling pathways in the liver

Arstikaitis, Jennifer; Gagné, François; Cyr, Daniel G. (2014). Exposure of fathead minnows to municipal wastewater effluent affects intracellular signaling pathways in the liver Comparative Biochemistry and Physiology. Toxicology and Pharmacology , vol. 164C . p. 1-10. DOI: 10.1016/j.cbpc.2014.04.002.

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Résumé

Municipal wastewater effluent can impact its receiving environment. In the St. Lawrence River, male fish living downstream from Montreal exhibit increased hepatic vitellogenin, intersex, delayed spermatogenesis and altered immune function. Few studies have examined genome-wide effects associated with municipal effluent exposure in fish to decipher the mechanisms of toxicity. The present objective was to identify hepatic cellular signaling pathways in fathead minnows following exposure to municipal wastewater effluent. Immature minnows were exposed for 21days to either 0% (Control) or 20% municipal effluent, the highest concentration in the St. Lawrence River. Hepatic RNA was extracted and used to hybridize a fathead minnow oligonucleotide microarray containing approximately 15k gene sequences. A total of 1300 genes were differentially expressed, of which 309 genes had more than 2-fold change in expression level between control and MWWE-exposed fish. Of those, 118 were up-regulated and 191 were down-regulated. Altered genes grouped according to function, indicated effects on various signaling pathways, apoptosis, immune responses, and cellular metabolism. Pathway analysis software predicted at least 5 signaling pathways that were altered by treatment: cell adhesion, inflammation, various kinases, estrogen receptor signaling and WNT signaling. Various components of the canonical Wnt pathway were dramatically down-regulated, while several other genes involved in the non-canonical Wnt pathway, such as Wnt4, LRP6, and PPP2R5E, which are known to inhibit the canonical Wnt pathway, were increased. These results indicate that municipal wastewater effluent from Montreal can target and inhibit various signaling including those implicated in hepatic Wnt signaling pathway in fathead minnows.

Type de document:
Mots-clés libres: Fathead minnow; Intracellular signaling; Liver; Microarray
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 29 mai 2017 20:31
Dernière modification: 29 mai 2017 20:31
URI: http://espace.inrs.ca/id/eprint/3005

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