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Melatonin: The watchdog of villous trophoblast homeostasis against hypoxia/reoxygenation-induced oxidative stress and apoptosis

Lanoix, Dave; Lacasse, Andrée-Anne; Reiter, Russel J; Vaillancourt, Cathy (2013). Melatonin: The watchdog of villous trophoblast homeostasis against hypoxia/reoxygenation-induced oxidative stress and apoptosis Molecular and Cellular Endocrinology , vol. 381 , nº 1-2. p. 35-45. DOI: 10.1016/j.mce.2013.07.010.

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Résumé

Human placenta produces melatonin and expresses its receptors. We propose that melatonin, an antioxidant, protects the human placenta against hypoxia/reoxygenation (H/R)-induced damage. Primary term villous cytotrophoblasts were cultured under normoxia (8% O2) with or without 1mM melatonin for 72h to induce differentiation into the syncytiotrophoblast. The cells were then cultured for an additional 22h under normoxia or subjected to hypoxia (0.5% O2) for 4h followed by 18h reoxygenation (8% O2) with or without melatonin. H/R induced oxidative stress, which activated the Bax/Bcl-2 mitochondrial apoptosis pathway and the downstream fragmentation of DNA. Villous trophoblast treatment with melatonin reversed all the negative effects induced by H/R to normoxic levels. This study shows that melatonin protects the villous trophoblast against H/R-induced oxidative stress and apoptosis and suggests a potential preventive and therapeutic use of this indolamine in pregnancy complications characterized by syncytiotrophoblast survival alteration.

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Mots-clés libres: Antioxidant; Mitochondrial apoptosis; Reactive oxygen species; Villous trophoblast
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 30 mai 2017 19:26
Dernière modification: 30 mai 2017 19:26
URI: http://espace.inrs.ca/id/eprint/2939

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