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The UL24 protein of herpes simplex virus 1 affects the sub-cellular distribution of viral glycoproteins involved in fusion

Ben Abdeljelil, Nawel; Rochette, Pierre-Alexandre; Pearson, Angela (2013). The UL24 protein of herpes simplex virus 1 affects the sub-cellular distribution of viral glycoproteins involved in fusion Virology , vol. 444 , nº 1-2. p. 263-73. DOI: 10.1016/j.virol.2013.06.021.

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Résumé

Mutations in UL24 of herpes simplex virus type 1 can lead to a syncytial phenotype. We hypothesized that UL24 affects the sub-cellular distribution of viral glycoproteins involved in fusion. In non-immortalized human foreskin fibroblasts (HFFs) we detected viral glycoproteins B (gB), gD, gH and gL present in extended blotches throughout the cytoplasm with limited nuclear membrane staining; however, in HFFs infected with a UL24-deficient virus (UL24X), staining for the viral glycoproteins appeared as long, thin streaks running across the cell. Interestingly, there was a decrease in co-localized staining of gB and gD with F-actin at late times in UL24X-infected HFFs. Treatment with chemical agents that perturbed the actin cytoskeleton hindered the formation of UL24X-induced syncytia in these cells. These data support a model whereby the UL24 syncytial phenotype results from a mislocalization of viral glycoproteins late in infection.

Type de document:
Mots-clés libres: F-actin; Herpes simplex virus 1; Syncytia; UL24; gB; gD
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 21 juin 2017 14:29
Dernière modification: 21 juin 2017 14:29
URI: http://espace.inrs.ca/id/eprint/2861

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