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Cyclic-di-GMP levels affect Pseudomonas aeruginosa fitness in the presence of imipenem

Nicastro, Gianlucca G.; Kaihami, Gilberto Hideo; Pereira, Thays O; Meireles, Diogo A.; Groleau, Marie-Christine; Déziel, Éric; Baldini, Regina L. (2014). Cyclic-di-GMP levels affect Pseudomonas aeruginosa fitness in the presence of imipenem Environmental Microbiology , vol. 16 , nº 5. p. 1321-1333. DOI: 10.1111/1462-2920.12422.

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Résumé

A large number of genes coding for enzymes predicted to synthesize and degrade 3′–5′-cyclic diguanylic acid (c-di-GMP) is found in most bacterial genomes and this dinucleotide emerged as an intracellular signal-controlling bacterial behaviour. An association between high levels of c-di-GMP and antibiotic resistance may be expected because c-di-GMP regulates biofilm formation and this mode of growth leads to enhanced antibiotic resistance. However, a clear understanding of this correlation has not been established. We found that increased levels of c-di-GMP in Pseudomonas aeruginosa improve fitness in the presence of imipenem, even when grown as planktonic cells. P. aeruginosa post-transcriptionally regulates the amounts of five porins in response to c-di-GMP, including OprD, responsible for imipenem uptake. Cells with low c-di-GMP levels are consequently more sensitive to this antibiotic. Main efflux pumps or β-lactamase genes did not show altered mRNA levels in P. aeruginosa strains with modified different c-di-GMP concentrations. Together, our findings show that c-di-GMP levels modulate fitness of planktonic cultures in the presence of imipenem.

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Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 29 avr. 2014 18:54
Dernière modification: 22 déc. 2016 19:23
URI: http://espace.inrs.ca/id/eprint/2181

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